Journal article
Inhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy
I Javed, G Peng, Y Xing, T Yu, M Zhao, A Kakinen, A Faridi, CL Parish, F Ding, TP Davis, PC Ke, S Lin
Nature Communications | NATURE RESEARCH | Published : 2019
Abstract
Alzheimer’s disease (AD) is the most prevalent form of neurodegenerative disorders, yet no major breakthroughs have been made in AD human trials and the disease remains a paramount challenge and a stigma in medicine. Here we eliminate the toxicity of amyloid beta (Aβ) in a facile, high-throughput zebrafish (Danio rerio) model using casein coated-gold nanoparticles (βCas AuNPs). βCas AuNPs in systemic circulation translocate across the blood brain barrier of zebrafish larvae and sequester intracerebral Aβ42 and its elicited toxicity in a nonspecific, chaperone-like manner. This is evidenced by behavioral pathology, reactive oxygen species and neuronal dysfunction biomarkers assays, complement..
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Awarded by National Science Foundation
Funding Acknowledgements
This work was supported by ARC Project No. CE140100036 (Davis), the NSFC grant #21607115 and #21777116 (Lin), NSF CAREER CBET-1553945 (Ding), and NIH MIRA R35GM119691 (Ding). Javed acknowledges the support of Monash International Postgraduate Research Scholarship (MIPRS) and Australian Government Research Training Program Scholarship. TEM imaging was performed at Bio21 Advanced Microscopy Facility, University of Melbourne and polarized light microscopy was performed at Monash Micro Imaging, Monash University. Javed and Lin thank Shanghai Science and Technology Commission "Belt and Road" initiative program, Grant no. 17230743000. The authors acknowledge the support from Profs. Ting Xu and Daqiang Yin on the Zebrabox instrument.